AgraQuant® Total Aflatoxin ELISA Test

AgraQuant® Total Aflatoxin test kits are accurate and reliable enzyme-linked immunosorbent assays (ELISA) in a quantitative format. Together with a StatFax® or BioTek® ELISA reader, results can be directly interpreted and continuously documented. These ELISA test kits are the ideal solution for a parallel measurement of multiple samples with incubation times of as low as 15 minutes for up to 42 samples. With the AgraQuant® ELISA, up to 6 mycotoxins can be analyzed from 1 extraction, substantially shortening overall time-to-result.

Materials supplied:

• antibody coated microwells in a microwell holder

• non-coated dilution microwells

• 5 or 6 vials of each aflatoxin standard

• 1 bottle of aflatoxin conjugate

• 1 bottle of substrate solution

• 1 bottle of stop solution

Materials required but not supplied

• Romer Series II® Mill or equivalent

• blender or a tightly sealing jar with lid

• balance - 400 g

• graduated cylinder: 100mL

• 100% methanol (ACS grade) methanol or 70% methanol 

• distilled or de-ionized water for preparing 70 % methanol

• 84% acetonitrile

• container with a minimum 125mL capacity

• Whatman#1 filter paper or equivalent

• filter funnel

• MycoSep 112 column for specific matrices

• 8-channel and single channel pipettes

• 100µL and 200µL with tips

• timer

• wash bottle

• absorbent paper towels

• 3 reagent boats for 8-channel pipettes

• microwell reader with a 450nm filter and a differential filter at 630 nm